Effect of carbon dioxide on the formation of α-amylase by Bacillus subtilis growing in continuous and batch cultures

✍ Scribed by A. P. Gandhi; L. Kjaergaard

• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 380 KB
• Volume: 17
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260170803

✦ Synopsis

The influence of COz on the formation of a-amylase by Bacillus subtilis NCIB 8646 growing in continuous and batch cultures was investigated.

Different levels of COz examined in the batch cultures stimulated the formation of a-amylase, with the highest activity being obtained using 6% COZ (v/v). The additions of C02 inhibit the growth and division of vegetative cells of B. subtilis when COZ is present in a concentration of more than 3y0 (v/v).

In chemostat cultures, air containing 8% COz (v/v) increased the specific enzyme productivity almost three times over the control, without affecting the cell growth.

An attempt is made to correlate the obtained results with the already established theory for the regulation of the a-amylase synthesis.