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Effects of ssDNA sequences on non-sequence-specific protein binding

โœ Scribed by Tung-Chung Mou; Michelle Shen; Saada Abdalla; Diego Delamora; Elena Bochkareva; Alexey Bochkarev; Donald M. Gray


Book ID
118282886
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
322 KB
Volume
18
Category
Article
ISSN
0899-0042

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Selection of High-Affinity Binding Sites
โœ S. Pierrou; S. Enerback; P. Carlsson ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 695 KB

We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble