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Effects of sodium phosphate buffer on horseradish peroxidase thermal stability

โœ Scribed by L. Haifeng; L. Yuwen; C. Xiaomin; W. Zhiyong; W. Cunxin


Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
247 KB
Volume
93
Category
Article
ISSN
0022-5215

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## Abstract Phthalic anhydride (PA) modification stabilizes horseradish peroxidase (HRP) by reversal of the positive charge on two of HRP's six lysine residues. Native and PAโ€HRP had halfโ€inactivation temperatures of 51 and 65ยฐC and halfโ€lives at 65ยฐC of 4 and 17 min, respectively. PAโ€HRP was more

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