✦ LIBER ✦

Effects of salts in promoting the adhesion of amphibian gastrula cells

✍ Scribed by Komazaki, Shinji

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 860 KB
Volume: 250
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402500106

No coin nor oath required. For personal study only.

✦ Synopsis

Presumptive ectodermal and endodermal cells were isolated from the gastrula of the newt Cynops pyrrhogaster, and the effects of salts on embryonic cell adhesion were examined. When the concentrations of NaC1, KC1, MgSO, and Ca (NO& in the culture medium were increased individually, they all effectively promoted ectodermal cell adhesion. MgSO, and Ca(NO& promoted endodermal cell adhesion, but NaCl and KC1 did not. The culture medium containing increased NaCl (90 mM) nonspecifically promoted ectodermal cell adhesion to the four substrata studiedpolystyrene dish, glass, collagen, and fibronectin. The effects of drugs that influence the membrane permeability of Na+ and Ca2' and the replacement of Na+, C1-, and Ca2+ with other ions were studied, The results show that the flow of Na ' and CaZ-into the cytoplasm promotes ectodermal cell adhesion but exerts no influence on endodermal cell adhesion.

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