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Nm23-H1 promotes adhesion of CAL 27 cells in vitro

✍ Scribed by Ružica Bago; Jasminka Pavelić; Gordana Maravić Vlahoviček; Maja Herak Bosnar

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
387 KB
Volume
48
Category
Article
ISSN
0899-1987

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✦ Synopsis

Abstract

nm23‐H1 was found to diminish metastatic potential of carcinoma cell lines and therefore was placed in the group of metastatic suppressor genes. Its protein product has a function of a nucleoside diphosphate kinase (NDPK) as well as protein kinase and nuclease. Though it was found that Nm23‐H1 is involved in many cellular processes, it is still not known how it promotes metastatic suppressor activity. Since the process of metastasis is dependent on adhesion properties of cells, the goal of our work was to describe the adhesion properties of CAL 27 cells (oral squamous cell carcinoma of the tongue) overexpressing FLAG/nm23‐H1. In our experiments, cells overexpressing nm23‐H1 show reduced migratory and invasive potential. Additionally, cells overexpressing nm23‐H1 adhere stronger on substrates (collagen IV and fibronectin) and show more spread morphology than the control cells. Results obtained by EGF induction of migration revealed that the adhesion strength predetermined cell response to chemoattractant and that Nm23‐H1, in this cell type, does not interfere with, EGF induced, Ras signaling pathway. These data contribute to the overall knowledge about nm23‐H1 and its role in cell adhesion, migration, and invasion, especially in oral squamous cell carcinoma. © 2009 Wiley‐Liss, Inc.

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