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Effects of salt and nickel ion on the conformational stability of Bacillus pasteurii UreE

✍ Scribed by Yeon-Hee Lee; Hyung-Sik Won; Mann-Hyung Lee; Bong-Jin Lee

Book ID
117104347
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
370 KB
Volume
522
Category
Article
ISSN
0014-5793

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The unfolding of shikimate kinase (SK) from Erwinia chrysanthemi by urea and its subsequent refolding on dilution of the denaturing agent has been studied in detail [Eur. J. Biochem. 269 (2002) 2124]. Comparison of the effects of urea on the enzyme with those of guanidinium chloride (GdmCl) and NaCl