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Effects of Salinity on Sperm Motility, Fertilization, and Development in the Pacific Herring, Clupea pallasi

✍ Scribed by Frederick J. Griffin, Murali C. Pillai, Carol A. Vines, Juha Kääriä, Thea Hibbard-Robbins, Ryuzo Yanagimachi and Gary N. Cherr

Book ID
124901826
Publisher
Marine Biological Laboratory
Year
1998
Tongue
English
Weight
955 KB
Volume
194
Category
Article
ISSN
0006-3185

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## Abstract Previously, we have demonstrated that sperm from the Pacific herring, __Clupea pallasi__, remain immotile upon spawning into the surrounding medium and initiate motility only upon contact with the micropyle area of the chorion (Yanagimachi et al., '92, Dev. Growth Differ., 34:447–461).

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## Abstract Pacific herring (__Clupea pallasi__) gametes were cryopreserved in a simple extender (15% dimethyl sulfoxide in herring Ringers). Sperm remained viable for over 7 months when kept at liquid nitrogen temperature. A rapid cooling rate by directly plunging into liquid nitrogen allowed a ma