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Effects of resolution, peak ratio and sampling frequency in diode-array fluorescence detection in liquid chromatography

✍ Scribed by Russel B. Poe; Sarah C. Rutan

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
842 KB
Volume
283
Category
Article
ISSN
0003-2670

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✦ Synopsis

In this work, different methods of quantification that use both fluorescence spectral and retention time data were evaluated for accuracy and precision in diode-array fluorescence detection in liquid chromatography. The methods evaluated include the peak height and peak area methods of quantification, an adaptive Kahnan filter and the generalized rank annihilation method. All of these methods were used to estimate the concentration of a model led txmponent in the presence of other partially resolved unmodelled components. The peak area and peak height methods of quantification gave the largest errors as the chromatographic resolution was decreased. The generalized rank annihilation method gave the most reliable results when the chromatographic resolution was greater than 0.3. However, this method was affected by retention time shifts between the calibration and unknown samples and the rate at which the spectra were acquired.

Keyworak Liquid chromatography; Accuracy; Chemometrics; Diode-array fluorescence detection

Over the past several years, recent advances in instrumentation have brought about changes in the way chromatography is performed and the way the resulting data are analyzed. With new detectors emerging, hyphenated methods such as GC-MS, GC-FTIR, LC-MS, LC-UV are becoming commonplace. However, with these new detectors comes an ever increasing amount of data. Traditional ways of analyzing data from these hyphenated methods do not fully utilize the additional information obtained from these instruments. Therefore, new data analysis methods must take into consideration the complexity and vast amount of data from these new methods.

There are several multivariate analysis meth-

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