Effects of recombinant plasmid size on cellular processes in Escherichia coli
โ Scribed by U.Eong Cheah; William A. Weigand; Benjamin C. Stark
- Book ID
- 119078925
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 614 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0147-619X
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The F' plasmids ORF-1 (purE+ tsxs proC+ lac+) and F'14 (argE+ metB+ ilv+) contain active regions of recombination, fre I and fre II correspondingly. The plasmid ORF-1 is stable in recF- cells (i.e., with the RecBC pathway of recombination) and decays in rec+ cells (RecBCF pathway) giving two types o