Effects of photostimulation on wound healing in diabetic mice

✍ Scribed by Yu, Wei; Naim, John O.; Lanzafame, Raymond J.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 578 KB
• Volume: 20
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/(sici)1096-9101(1997)20:1<56::aid-lsm9>3.0.co;2-y

✦ Synopsis

Background and objective:

Low-level laser irradiation at certain fluences and wavelengths can enhance the release of growth factors from fibroblasts and stimulate cell proliferation in vitro. we evaluated whether low-level laser irradiation can improve wound healing in diabetes mellitus.

Study design/materials and methods:

Genetically diabetic mice (c57bl/ksj/db/db) were used as the animal model for this wound healing study. the experimental animals were divided among four groups: negative control, positive control (topical basic fibroblast growth factor [bfgf] on wound), laser therapy group; and a combination group of laser therapy and topical bfgf. an argon dye laser (lexel auora model 600) at a wavelength of 630 nm and an output of 20 m w/cm2 was used as the light source. the speed of wound closure and histological evaluation were used to analyze the experimental results.

Results:

Laser irradiation enhanced the percentage of wound closure over time as compared to the negative control group (58.4 +/- 2.6 vs. 40.8 +/- 3.4 at day 10 and 95.7 +/- 2 vs. 82.3 +/- 3.6 at day 20, p < .01). histological evaluation showed that laser irradiation improved wound epithelialization, cellular content, granulation tissue formation, and collagen deposition in laser-treated wounds as compared to the negative control group (6.4 +/- 0.16 vs. 3.8 +/- 0.13 at day 10 and 12 +/- 0.21 vs. 8.2 +/- 0.31, p < .01).

Conclusion:

This study of laser biostimulation on wound healing in diabetic mice suggests that such therapy may be of great benefit in the treatment of chronic wounds that occur as a complication of diabetes mellitus.