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Effects of pH of Fermentation Medium on Biosynthesis of Poly[(3-hydroxybutyrate)-co-(3-mercaptopropionate)] by Wautersia eutropha

✍ Scribed by Yuichi Isemori; Koichiro Tajima; Syogo Tanaka; Fang Yu; Kazuki Ishida; Yoshio Inoue

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 146 KB
Volume: 6
Category: Article
ISSN: 1616-5187
DOI: 10.1002/mabi.200600133

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✦ Synopsis

Abstract

Summary: A series of P(3HB‐co‐3MP)s with different 3MP unit content was biosynthesized by the fermentation of W. eutropha in a medium containing sodium gluconate and DTDP as carbon sources at different pH conditions ranging from pH 6.0 to 8.0. The P(3HB‐co‐3MP) samples were fractioned using the solvent/nonsolvent mixed solvent chloroform/heptane and the comonomer unit composition was investigated. It was found that W. eutropha produces P(3HB‐co‐3MP)s with extremely different 3MP unit content ranging from 3.6 to 70.0 mol‐%, depending on the pH value of the fermentation medium. The copolyester samples produced in mild basic medium have a considerably narrower compositional distribution than the samples from acidic medium. The highest polymer yield was obtained at pH 8.0.

DSC diagram for P(3HB‐co‐3MP)s biosynthesized in different pH medium.

magnified imageDSC diagram for P(3HB‐co‐3MP)s biosynthesized in different pH medium.

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