Biosynthesis and Compositional Regulation of Poly[(3-hydroxybutyrate)-co-(3-hydroxyhexanoate)] in Recombinant Ralstonia eutropha Expressing Mutated Polyhydroxyalkanoate Synthase Genes
✍ Scribed by Takeharu Tsuge; Yu Saito; Yoshihiro Kikkawa; Tomohiro Hiraishi; Yoshiharu Doi
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 100 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1616-5187
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Summary: A new strategy for bacterial polyhydroxyalkanoate (PHA) production by recombinant Ralstonia eutropha PHB^−^4 harboring mutated PHA synthase genes (phaC~Ac~) from Aeromona caviae was investigated. The strain harboring wild‐type phaC~Ac~ gene produced a PHA copolymer consisting of (R)‐3‐hydroxybutyrate and (R)‐3‐hydroxyhexanoate [P(3HB‐co‐3HHx)] with 3.5 mol‐% of 3HHx fraction from soybean oil. When the mutants of phaC~Ac~ gene were applied to this production system, 3HHx fraction in copolymers was varied in the range of 0–5.1 mol‐%. Thus, the regulation of PHA copolymer compositions has been achieved by the use of mutated PHA synthase genes.
Relationship between PHA synthase activity and 3HHx fraction in P(3HB‐co‐3HHx).
imageRelationship between PHA synthase activity and 3HHx fraction in P(3HB‐co‐3HHx).