It is suggested that the somatic recombination observed in Bloom's syndrome and cells treated with mitomycin C may be the result of selection for recombination events that can occur only between homologous segments of DNA, rather than a result of somatic pairing in the nucleus.
Effects of mitomycin C on sister chromatid exchange in normal and bloom's syndrome cells
β Scribed by Yukimasa Shiraishi; Avery A. Sandberg
- Book ID
- 113191392
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 362 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0027-5107
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Sister chromatid exchange (SCE) has been studied in the fibroblasts of five Bloom's syndrome patients, one heterozygote, and two normal individuals. The high frequency of SCE already known in the lymphocytes of Bloom's syndrome was also found in the fibroblasts of all five patients. However, populat
Cocultivation of fibroblast cells from a male patient with Bloom syndrome (BS) and a female control reduced the rate of sister chromatid exchanges in the BS cells from a mean of 54 SCE per metaphase (range 42--65) to 41 (range 24--59). Medium used to culture control cells for 48 h also reduced the r
A delay in cell cycle progression and a decrease in sister chromatid exchange (SCE) frequency was observed in Bloom syndrome (BS) cells when exposed to polyethylene glycol (PEG) treatment followed by co-cultivation with unexposed normal cells, whereas the SCE rate of both normal and BS cells increas