Effects of magnesium and temperature on the conformation and reassociation of Escherichia coli and Sulfolobus solfataricus ribosomes
โ Scribed by F Pedone; A Bonincontro; G Briganti; A Giansanti; P Londei; G Risuleo; M Mengoni
- Book ID
- 117481475
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 124 KB
- Volume
- 1335
- Category
- Article
- ISSN
- 0304-4165
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The effect of temperature on the synthesis of ribosome in Escherichia coli K-12 was investigated. In continuous fermentation, the total and functioning ribosome contents decreased with increasing temperature, while the nonfunctioning ribosome content remained unchanged. Cells contained higher amount
Reversible change of 50 S ribosomal subunits to 40 S particles takes place in cold buffered 0.5 M NH4C1 solutions either containing Mg ยง247 (up to 0.1 M), or free from Mg ~ and even supplemented with EDTA (1 mM). The 40 S particles were stable only within a definite temperature range. Heating of the