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Effects of homocysteine on metabolic pathways in cultured astrocytes

✍ Scribed by Ying Jin; Lorraine Brennan

Book ID
116765161
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
398 KB
Volume
52
Category
Article
ISSN
0197-0186

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## Abstract Cortical and cerebellar astrocytes were cultured in medium containing pentylenetetrazole (PTZ), a γ‐aminobutyric acid (GABA)~A~ receptor antagonist, for 3 weeks (up to 6 mM) or 2 hr (10 mM). Cells were incubated in medium containing [U‐^13^C]glutamate (0.5 mM) and unlabeled glucose (3 m