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Effects of heparin on polymerase chain reaction for blood white cells

✍ Scribed by Masaharu Yokota; Noriyuki Tatsumi; Oytip Nathalang; Tetsuaki Yamada; Izumi Tsuda

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
238 KB
Volume
13
Category
Article
ISSN
0887-8013

No coin nor oath required. For personal study only.

✦ Synopsis

Polymerase chain reaction (PCR) has been used with increasing frequency to diagnose infectious and genetic diseases. In this study, the effects of heparin on PCR were investigated, because heparinized blood may sometimes be used in PCR studies. HLA-DQA1 gene amplification was used as a model. PCR was clearly interfered with when heparinized blood was used as a source of template DNA, and the degree of interference was affected by the following three factors; (1) type of Taq DNA polymerase; (2) leukocyte count in blood; and

(3) concentration of heparin contained. When additional tests were conducted with additions of definite heparin concentrations to a PCR reaction mixture, specimens with large amounts of DNA tended to exhibit less interference by heparin. The addition of β‰₯ 0.1 to 0.0016 U of heparin per reaction mixture (50 Β΅l) suppressed DNA amplification in a dose-dependent fashion. We therefore concluded that much care should be taken when heparinized blood is used as a PCR material.

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