Effects of Heat Shock on Nuclear and Nucleolar Protein Phosphorylation in Chinese Hamster Ovary Cells
✍ Scribed by Michèle CAIZERGUES-FERRER; Gérard BOUCHE; François AMALRIC; Jean-Pierre ZALTA
- Book ID
- 115118844
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 894 KB
- Volume
- 108
- Category
- Article
- ISSN
- 1432-1327
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
The possible mechanism for heat protection by the protein synthesis inhibitor histidinol was investigated in CHO cells. Histidinol (HST, 5 mM), an analogue of the essential amino acid L-histidine, added for 2 hr before and during heating at 43"C, protected cells from killing at 43°C. Treatment with
During 4 hr after puromycin (PUR: 20 pg/ml) treatment, the synthesis of three major heat shock protein families (HSPs: M, = 110,000,87,000, and 70,000) was enhanced 1.5-fold relative to that of untreated cells, as studied by one-dimensional gel electrophoresis. The increase of unique HSPs, if studie
In the absence of serum and amino acids, cultured Chinese Hamster Ovary cells released to the medium two thirds of the leucine produced by protein degradation. Because protein synthesis requires all the amino acids, the loss of leucine implies incomplete reincorporation of the other amino acids as w