Effects of glycine antagonists in an acute in vitro electrophysiological model of ischemia

✍ Scribed by Donald E. Schilp; Stephen M. Sorensen; Joseph G. Wettstein; Mark D. Black

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 118 KB
• Volume: 46
• Category: Article
• ISSN: 0272-4391
• DOI: 10.1002/(sici)1098-2299(199902)46:2<134::aid-ddr6>3.0.co;2-9

✦ Synopsis

The effects of several glycine antagonists at the strychnine-insensitive site on N-methyl-Daspartate (NMDA) receptors in an acute in vitro electrophysiological model of ischemia in the rat hippocampus were studied. Hippocampal slices were subjected to 9 min of hypoxia and hypoglycemia (ischemia). The noncompetitive NMDA antagonist MK-801 produced significant protection of the slices, whereas the competitive NMDA antagonist D-CPP did not. Administration of the strychnine-insensitive glycine-modulatory site antagonists (ACEA 1021, GV150,526A, and L701,324) up to 10 µM did not result in significant recovery of function. Other animal models have suggested that glycine antagonists are beneficial 6-48 hours after ischemic insult; this model assayed functional recovery for only 1 hour after the insult. Therefore, we conclude that this acute in vitro electrophysiologic model may not be useful in detecting the potential neuroprotective properties of glycine antagonists.