Effects of elicitors on the enhancement of asiaticoside biosynthesis in cell cultures of centella (Centella asiaticaL. Urban)

✍ Scribed by Loc, Nguyen Hoang; Giang, Nguyen Thanh

• Book ID: 115063885
• Publisher: Versita
• Year: 2012
• Tongue: English
• Weight: 277 KB
• Volume: 66
• Category: Article
• ISSN: 0366-6352
• DOI: 10.2478/s11696-012-0168-9

✦ Synopsis

Abstract

In this work, the effects of elicitor concentration and elicitation day on the growth and asiaticoside production of centella cells were investigated. The results showed that 2-hydroxybenzoic acid from 50–200 μM and yeast extract from 2–5 g L−1 had different eliciting influences. The addition of 2-hydroxybenzoic acid and yeast extract to the cultures strongly enhanced asiaticoside production in centella cells. The increase in asiaticoside content induced by the addition of 100 μM of 2-hydroxybenzoic acid and 4 g L−1 of yeast extract at day 10 of inoculation was about 5- and 3.5-fold, respectively, as compared with that of the reference cells. In general, 2-hydroxybenzoic acid (abiotic elicitor) was more effective in enhancing asiaticoside biosynthesis than yeast extract (biotic elicitor).