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Effects of electrical fields on cardiomyocyte differentiation of embryonic stem cells

✍ Scribed by Heinrich Sauer; Gohar Rahimi; Jürgen Hescheler; Maria Wartenberg

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
685 KB
Volume
75
Category
Article
ISSN
0730-2312

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✦ Synopsis

The effects of electromagnetic fields (EMFs) on the differentiation of cardiomyocytes in embryoid bodies derived from pluripotent embryonic stem (ES) cells were investigated. A single direct current (DC) field pulse was applied to 4-day-old embryoid bodies. The electrical field induced a hyperpolarization of the anode-facing side of embryoid bodies and a depolarization at the cathode-facing side. Significant effects of a single electrical field pulse applied for 90 s on cardiomyocyte differentiation were achieved with field strengths of 250 and 500 V/m, which increased both the number of embryoid bodies differentiating beating foci of cardiomyocytes and the size of the beating foci. The 500-V/m electrical field increased intracellular reactive oxygen species (ROS), but not [Ca 2ϩ ] i and activated nuclear factor kappa B (NF-B). A comparable increase in the number of beating embryoid bodies was achieved by an incubation for 1 h with H 2 O 2 (1-10 nM), indicating that the electrical field effect was transduced via the intracellular generation of ROS. Because the radical scavengers dehydroascorbate and pyrrolidinedithiocarbamate (APDC) and the NF-B antagonist N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) inhibited cardiac differentiation, we assume that ROS and NF-B may play a role in early cardiac development.

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