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Effects of cultural conditions on alkaline phosphatase and cell culture survival in the presence of prednisolone

✍ Scribed by G. Melnykovych; M.A. Swayze; C.F. Bishop


Book ID
118875125
Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
542 KB
Volume
47
Category
Article
ISSN
0014-4827

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In two heteroploid cell lines synchronized with thymidine double block, activity of alkaline phosphatase decreased during the 12 hour period preceding mitotic peak. A return to high values was observed during the next 12 hours of synchronous cycle. Prednisolone ( llp, 17a, 21-trihydroxy-1,4-pregnadi

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## Abstract The effects of different culture conditions, suspension and microcarrier culture and temperature reduction on the structures of N‐linked glycans attached to secreted human placental alkaline phosphatase (SEAP) were investigated for CHO cells grown in a controlled bioreactor. Both mass s