𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effects of cryopreservation on CD4+ CD25+ T cells of HIV-1 infected individuals

✍ Scribed by Anna C. Seale; Bouke C. de Jong; Irfan Zaidi; Melody Duvall; Hilton Whittle; Sarah Rowland-Jones; Assan Jaye

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
147 KB
Volume
22
Category
Article
ISSN
0887-8013

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The role of T regulatory cells (Tregs) in human immunodeficiency virus (HIV)‐1 infection, although not entirely clear, has recently been highlighted. Despite their lack of specificity, fluorochrome‐labeled CD4 and CD25 antibodies are common flow cytometric reagents used to identify these cells with immunosuppressive potential. Cryopreservation has previously been shown to alter the proportions of lymphocytes with certain phenotypes expressed in peripheral blood mononuclear cells (PBMCs). The aim of this study was to assess the effect of cryopreservation on CD4+ CD25+ T cells in PBMCs from HIV‐1+ individuals to guide the design of future studies on Tregs. We recruited 30 HIV‐1+ individuals and nine healthy controls. CD25 expression in CD4+ T cells was compared between fresh and frozen/thawed PBMC samples from the same time point. In this study, cryopreservation significantly decreased the proportion of CD4+ CD25+ T cells in PBMC samples from HIV‐1 infected subjects. This finding suggests that studies of CD4+ CD25+ T cells should be carried out on fresh samples to avoid bias introduced by cryopreservation. J. Clin. Lab. Anal. 22:153–158, 2008. Β© 2008 Wiley‐Liss, Inc.

πŸ“œ SIMILAR VOLUMES

Quantitation of CD38 activation antigen
Quantitation of CD38 activation antigen expression on CD8+ T cells in HIV-1 infection using CD4 expression on CD4+ T lymphocytes as a biological calibrator
✍ Lance E. Hultin; Jose L. Matud; Janis V. Giorgi πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 131 KB πŸ‘ 2 views

For some membrane-associated antigens, the number of molecules expressed per cell carries information about the cell's differentiation and activation state. Quantitating antigen expression by flow cytometry has immediate application in monitoring CD38 expression on CD8 Ψ‰ T cells in human immunodefic

Role of CD95-activated caspase-1 process
Role of CD95-activated caspase-1 processing of IL-1Ξ² in TCR-mediated proliferation of HIV-infected CD4+ T cells
✍ FrΓ©dΓ©ric Petit; Jacques Corbeil; Jean-Daniel LeliΓ¨vre; Laure Moutouh-de Parseval πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 323 KB

CD95 plays a critical role in the homeostasis of the immune system, and has been reported to participate in T cell death during HIV infection. Here we report that the response to CD3-TCR stimulation of CD4 + T cells from HIV-infected individuals and CD4 + T cells from healthy donors incubated in vit

Cell cycle distribution of CD4+ lymphocy
Cell cycle distribution of CD4+ lymphocytes in HIV-1-infected subjects
✍ Chaves, Mariana Melillo Sauer ;Kallas, Esper Georges πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 221 KB

## Abstract ## Background Apoptosis is one of the possible explanations for the progressive loss of CD4^+^ T lymphocytes in infection with the human immunodeficiency virus (HIV), which may interfere with cell cycle distribution. This study evaluated the cell cycle of CD4^+^ and CD8^+^ lymphocytes