𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effects of chemical modifications in the partition behavior of proteins in aqueous two-phase systems: A case study with RNase A

✍ Scribed by González-Valdez, José; Rito-Palomares, Marco; Benavides, Jorge


Book ID
120344674
Publisher
American Institute of Chemical Engineers
Year
2013
Tongue
English
Weight
248 KB
Volume
29
Category
Article
ISSN
8756-7938

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Conservative chemical modification of pr
✍ T. T. Franco; A. T. Andrews; J. A. Asenjo 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 933 KB

Relatively conservative modifications of three proteins were carried out to alter their surface properties. The protein properties modified were hydrophobicity and charge. This was done by acylation of amino groups with anhydrides. For the hydrophobic modification experiments, two proteins (p-lactog

Correlations for the partition behavior
✍ Anette S. Schmidt; Barbara A. Andrews; Juan A. Asenjo 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 899 KB

The effect of protein concentration in partitioning in PEG/ salt aqueous two-phase systems has been investigated. PEG 4000lphosphate systems in the presence of 0% wlw and 8.8% wlw NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000lphosphate system wi

Use of chemically modified proteins to s
✍ T. T. Franco; A. T. Andrews; J. A. Asenjo 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 867 KB

Two different series of hydrophobically modified proteins were partitioned in a number of aqueous two-phase systems (ATPS) to investigate the effect of hydrophobicity as a single property on partitioning. The modified proteins were derived from P-lactoglobulin and bovine serum albumin (BSA). Measure

Use of chemically modified proteins to s
✍ T. T. Franco; A. T. Andrews; J. A. Asenjo 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 693 KB

A series of charge-modified thaumatins with different values of surface charge were partitioned in aqueous twophase systems (ATPS) to study the effect of surface charge as a single property on partitioning. Electrophoretic mobility of the proteins in titration curves was used as a measure of surface

Chemical modification of chicken egg whi
✍ Yoshitsune Shin-ya; Jin-Won Park; Kyung-Jin Hong; Koichi Fujiwara; Toshio Kajiuc 📂 Article 📅 2005 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 127 KB

## Abstract Aqueous two‐phase partitioning is one of the excellent bioseparation systems for proteins and other biomolecules in water‐rich solutions containing polymers or salts. To improve the partition properties of model proteins, chicken egg white (CEW) proteins were chemically modified with po

Correlation for the partition behavior o
✍ B.A. Andrews; A.S. Schmidt; J.A. Asenjo 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 566 KB

## Abstract Correlations to describe the effect of surface hydrophobicity and charge of proteins with their partition coefficient in aqueous two‐phase systems were investigated. Polyethylene glycol (PEG) 4000/phosphate, sulfate, citrate, and dextran systems in the presence of low (0.6% w/w) and hig