Effects of bacillus Calmette-Guérin and interferon-α-2B on human bladder cancer in vitro

✍ Scribed by Ye Zhang; Hoon Eng Khoo; Kesavan Esuvaranathan

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: French
• Weight: 160 KB
• Volume: 71
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19970529)71:5<851::aid-ijc25>3.0.co;2-9

✦ Synopsis

The cytolytic and anti-proliferative effects of bacillus Calmette-Gue ´rin (BCG) and/or interferon-a-2b (IFN-a-2b) on 5 human bladder carcinoma cell lines, RT4, RT112, MGH, SD and J82, were determined. The cell lines showed different sensitivities to BCG and IFN-a-2b. BCG had direct dosedependent cytolytic and anti-proliferative effects on MGH, J82 and SD (grade 3 cell lines), whereas RT4 and RT112 (grades 1 and 2, respectively) were less sensitive. Surprisingly, higher concentrations of BCG enhanced cell growth of RT4. IFN-a-2b also had cytolytic and anti-proliferative effects on all 5 cell lines. Thus, the RT4 and RT112 cell lines that were not sensitive to BCG were highly sensitive to IFN-a-2b. A combination of BCG and IFN-a-2b had additive anti-proliferative effects on MGH, J82 and RT112. Interleukin-6 (IL-6) and tumor necrosis factor-a (TNF-a) production by these 5 cell lines was measured after stimulation with BCG and/or IFN-a-2b, by ELISA immunoassays. Production of IL-6 and TNF-a was significantly increased in MGH and J82 cell lines by the combination of BCG and IFNa-2b. The enhanced cytolytic and anti-proliferative effects of the combination of BCG and IFN-a-2b may be related to the induction of cytokines. Int.