Effects of aspirin on arylamine N-acetyltransferase activity and DNA adducts in human bladder tumour cells

✍ Scribed by Chin-Chung Yeh; Jing-Gung Chung; Hsi-Chin Wu; Huei-Ling Chang; Feng-Tsgh Yeh; Chi-Fu Hung

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 55 KB
• Volume: 19
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/(sici)1099-1263(199911/12)19:6<389::aid-jat596>3.0.co;2-d

✦ Synopsis

Aspirin; N-acetyltransferase; 2-aminofluorene; DNA adduct; bladder cancer Aspirin (acetylsalicylic acid) was used to determine the inhibition of arylamine N-acetyltransferase (NAT) activity and DNA adduct formation in a human bladder tumour cell line (T24). The activity of NAT was measured by high-performance liquid chromatography, assaying for the amounts of N-acetyl-2-aminofluorene and N-acetyl-p-aminobenzoic acid and remaining 2-aminofluorene and p-aminobenzoic acid. Two assay systems were used: one with cytosol and the other with intact cells. High-performance liquid chromatography was also used to analyse for the 2-aminofluorene-DNA adducts. Intact bladder tumour cells were used. The results demonstrated that NAT activity and 2-aminofluorene-DNA adduct formation in human bladder tumour cells were inhibited by acetylsalicylic acid in a dose-dependent manner. The effects of acetylsalicylic acid on the values of the apparent K m and V max were also determined in both examined systems. The data also indicated that acetylsalicylic acid decreased the apparent values of K m and V max from human bladder tumour cells in both cytosol and intact cells.