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Effects of Ammonia and Glucosamine on the Heterogeneity of Erythropoietin Glycoforms

✍ Scribed by M. Yang; M. Butler

Book ID
109386363
Publisher
American Institute of Chemical Engineers
Year
2002
Tongue
English
Weight
241 KB
Volume
18
Category
Article
ISSN
8756-7938

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Effects of ammonia on CHO cell growth, e
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✍ M. Yang; M. Butler πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 258 KB πŸ‘ 3 views

The effect of ammonium chloride was determined on a culture of CHO cells transfected with the human erythropoietin (EPO) gene. Cell growth was inhibited above a culture concentration of 5 mM NH 4 Cl with an IC-50 determined to be 33 mM. The specific production of EPO increased with the addition of N