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Effect of Ammonia on the Glycosylation of Human Recombinant Erythropoietin in Culture

✍ Scribed by M. Yang; M. Butler

Book ID
109386154
Publisher
American Institute of Chemical Engineers
Year
2000
Tongue
English
Weight
285 KB
Volume
16
Category
Article
ISSN
8756-7938

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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37Β°C until viable cell concentration reached 1 × 10^7^ c

Effects of ammonia on CHO cell growth, e
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The effect of ammonium chloride was determined on a culture of CHO cells transfected with the human erythropoietin (EPO) gene. Cell growth was inhibited above a culture concentration of 5 mM NH 4 Cl with an IC-50 determined to be 33 mM. The specific production of EPO increased with the addition of N