Effect of vanadate on brain protein phosphorylation
✍ Scribed by Jiří Křivánek
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 626 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0364-3190
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Proteins phosphorylated at tyrosine residues in the developing rat brain have been identified with a focus on the nerve growth cone and synaptic terminal. Endogenous protein phosphorylation in membranes from a subcellular growth cone fraction of fetal rat brain revealed prominent 55-60 kD phosphotyr
enough for the characterization of cationic lipid-mediated gene transfer in various adherent cells. If an increase of sensitivity is required, either buffers other than HBSS and Tris-HCl can be used or a higher incubation temperature can be employed. Nevertheless, we found this assay highly suitable