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Effect of Ultrasound on DNA Polymerase Reactions: Monitoring on a 27-MHz Quartz Crystal Microbalance

โœ Scribed by Hoshino, Yu; Kawasaki, Takayoshi; Okahata, Yoshio


Book ID
127317536
Publisher
American Chemical Society
Year
2006
Tongue
English
Weight
149 KB
Volume
7
Category
Article
ISSN
1525-7797

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Catalytic reactions of DNA polymerase I from E. coli (Klenow fragment, KF) were monitored directly with a template/primer (40/25-or 75/25-mer)immobilized 27-MHz quartz-crystal microbalance (QCM). The 27-MHz QCM is a very sensitive mass-measuring device in aqueous solution, as the frequency decreases