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Direct Monitoring of DNA Polymerase Reactions on a Quartz-Crystal Microbalance

โœ Scribed by Niikura, Kenichi; Matsuno, Hisao; Okahata, Yoshio

Book ID
127230591
Publisher
American Chemical Society
Year
1998
Tongue
English
Weight
38 KB
Volume
120
Category
Article
ISSN
0002-7863

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Direct Monitoring Kinetic Studies of DNA
Direct Monitoring Kinetic Studies of DNA Polymerase Reactions on a DNA-Immobilized Quartz-Crystal Microbalance
โœ Hisao Matsuno; Kenichi Niikura; Yoshio Okahata ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 187 KB ๐Ÿ‘ 1 views

Catalytic reactions of DNA polymerase I from E. coli (Klenow fragment, KF) were monitored directly with a template/primer (40/25-or 75/25-mer)immobilized 27-MHz quartz-crystal microbalance (QCM). The 27-MHz QCM is a very sensitive mass-measuring device in aqueous solution, as the frequency decreases