Effect of the leader sequence on the expression of recombinantC. rugosalipase byS. cerevisiaecells

✍ Scribed by Fabrizia Fusetti; Stefania Brocca; Danilo Porro; Marina Lotti

• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 380 KB
• Volume: 18
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00142945

✦ Synopsis

The Candida rugosa lipase I gene has been expressed in Saccharomyces cerevisiae. The recombinant lipase was efficiently synthesized only following the replacement of the enzyme endogenous leader sequence with the signal peptide of the Kluyveromyces lactis killer toxin. Amount of accumulated lipase was about 10-20 mg/l in batch culture and over lgfl in a computer-controlled fed-batch fermentation system.

INTRODUCI'ION

Lipases are ester hydrolases able to catalyze the breakdown of ester bonds in insoluble substrates, such as natural oils and fats, cholesterol esters and intermediates for chemical synthesis. Their ability to catalyze the reverse synthetic reaction in low water media and their