The introduction of an inverted repeat to the 5′ untranslated leader sequence of a transgene strongly inhibits gene expression

The 5′-untranslated leader sequence of p

The 5′-untranslated leader sequence of potato virus X RNA enhances the expression of a heterologous gene in vivo

✍ Pooggin, Mikhail M. ;Skryabin, Konstantin G. 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 366 KB

The 5' untranslated leader of potato virus X (PVX) RNA is shown when contiguous to the coding sequence, to enhance the expression of the neomycin phosphotransferase II gene (NPTII) in Nicotiana tabacum protoplasts in vivo. The level of transient expression of the NPTII gene in protoplasts provided b