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Effect of the intracellular localization of a Gd-based imaging probe on the relaxation enhancement of water protons

✍ Scribed by Enzo Terreno; Simonetta Geninatti Crich; Simona Belfiore; Luigi Biancone; Claudia Cabella; Giovanna Esposito; Andrea D. Manazza; Silvio Aime

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
267 KB
Volume
55
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

Gd‐HPDO3A has been internalized into rat hepatocarcinoma cells in the cytoplasm (by electroporation) or in intracellular vesicles (by pinocytosis), respectively. In the former case, the observed relaxation rates are likely dependent upon the amount of internalized paramagnetic complex, whereas in the latter case the relaxation enhancement is “quenched” to a plateau value (about 3 s^−1^) when the entrapped amount of Gd‐chelate is higher than 1 × 10^10^ Gd/cell. The observed behavior has been accounted in terms of a theoretical treatment based on equations formally derived by Labadie et al. (J Magn Reson B 1994;105:99–102). On this basis, entrapment into intracellular vesicles has been treated as a three‐site water exchange (extracellular/cytoplasm/vesicle compartments), whereas the cell pellets containing the paramagnetic agent spread out in the cytoplasm can be analyzed by a two‐site exchange system. Magn Reson Med, 2006. © 2006 Wiley‐Liss, Inc.

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