Effect of temperature shock and elevated incubation temperature on androgenic embryo yield of diploid potato

✍ Scribed by Liu Yin Shen; Richard E. Veilleux

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 540 KB
• Volume: 43
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00042668

✦ Synopsis

Using three diploid tuber-bearing Solanum clones as anther donors, experiments were conducted on the effect of high temperature shock and elevated incubation temperature during anther culture on androgenic embryo production. Five incubation treatments were tested on two clones and three treatments were repeated in a second experiment on one of the same clones and an additional one. In the first experiment, temperature treatment, genotype, date of culture initiation, and their interactions were all significant sources of variation. A treatment combining a high temperature shock (35 °C for 12 h) with elevated incubation temperature (30/20 °C) yielded 11 times as many embryos (44 per flask) as the control 20 °C (4 per flask). By conducting several replications per day of bud collection, the significant variation due to experimental dates was separated from experimental error to provide a more sensitive test of treatment effects. Temperature shock (35 °C 12h) during anther culture did not appear to influence the subsequent conversion rate of androgenic embryos.