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Effect of temperature on plasmid stability and expression of cloned cellulase gene in a recombinantBacillusmegaterium

✍ Scribed by Kwang H. Son; Jong H. Jang; Jung H. Kim

Publisher
Springer Netherlands
Year
1987
Tongue
English
Weight
229 KB
Volume
9
Category
Article
ISSN
0141-5492

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The btuB gene of Escherichia coli codes for a protein (BtuB) located in the outer membrane. BtuB is the receptor for vitamin B12 (cyanocobalamin). We have cloned the btuB gene into pUC8 using transposon Tn5 as the marker to first isolate several parts of the relevant DNA fragment from the specialize