Effect of temperature on early embryological development of the zebra fish,Brachydanio rerio

✍ Scribed by Schirone, Robert C. ;Gross, Leo

• Publisher: John Wiley and Sons
• Year: 1968
• Tongue: English
• Weight: 662 KB
• Volume: 169
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1401690106

✦ Synopsis

The early development of the zebra fish embryo, Brachydanio rerio (Hamilton) was studied under thermostatically controlled temperatures ranging from 13" to 35°C. Regular and successive cleavages and a distinct continuity of morphological features proceed regularly between 23" and 34°C. Embryos kept at temperatures above or below these limits did not complete embryogenesis. Embryogenesis up to late high blastula proceeded at the same rate of development over the temperature range of 24°C to 32°C. Time-lapse cinephotomicrography was utilized in a study of development from the one-celled blastodisc to the closure of the blastopore, incubated in a modified Rose culture chamber. The number of abnormal embryos was constant over the range of temperatures from 23" to 34°C.