✦ LIBER ✦

EFFECT OF STEROID HORMONES ON MEMBRANE PROFILES OF HELA S3 CELLS

✍ Scribed by P. Sommer; H.M. Cowley

Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 143 KB
Volume: 25
Category: Article
ISSN: 1065-6995
DOI: 10.1006/cbir.2000.0682

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The cervical carcinoma cell line, HeLa S3, was exposed to oestradiol and progesterone separately and combined in ratios similar to those of the luteal and follicular phases of the menstrual cycle. After 48h of exposure, membrane proteins were extracted from the plasma membrane and analyzed. The lipid composition of the cells and lectin binding patterns to the cells were also assessed. The hormones had a profound effect on the HeLa S3 membrane protein profiles. Progesterone inhibited the synthesis of a wide variety of proteins while oestradiol negated this effect. Less variation between lipid profiles was noted although progesterone reduced the amount of cholesterol present. Differences in lectin binding profiles between the various treatments were noted, indicating an hormonal effect on glycosylation. Such hormonal effects on membrane composition may have implications in cervical susceptibly to microbial infections.

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