An automated high-performance liquid chromatographic method has been developed that allows for the determination of a number of compounds related to catechol- and indoleamine metabolism. The compounds that can be measured include L-DOPA, dopamine, dihydroxyphenylacetic acid, homovanillic acid, 3-met
Effect of some parameters on the liquid chromatographic separation of biogenic amines and their metabolites
β Scribed by Lammers, N. ;Zeeman, J. ;De Jong, G. J.
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 274 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0935-6304
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β¦ Synopsis
Abstract
The liquid chromatographic separation of some biogenic amines and their metabolites with a reversedβphase ionβpair system has been optimized. The effects of the nature of the stationary phase, the column temperature, the pH, and the ionβpair reagent concentration of the mobile phase have been investigated. The most striking result is the influence of the column temperature on selectivity.
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