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Effect of some organic cosolvents on the reaction of hemoglobin with oxygen

✍ Scribed by Lorenzo Cordone; Antonio Cupane; Pier L. San Biagio; Eugenio Vitrano

Publisher
Wiley (John Wiley & Sons)
Year
1981
Tongue
English
Weight
659 KB
Volume
20
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

We studied the effects of some organic cosolvents (monohydric alcohols and amides) on the reaction of hemoglobin with oxygen. We present evidence showing that our data can be analyzed within the framework of the Monod‐Wyman‐Changeux model and that the main effect of cosolvents is to alter the T ⇄ R conformational equilibrium of hemoglobin, without significantly affecting the intrinsic oxygen dissociation constants. Following a previously described phenomenological approach, the overall effects have been separated into effects related to the variation of the bulk dielectric constant of the solvent and effects not related to the variation of this constant.

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