Effects of some organic cosolvents on the functional properties of hemoglobin: Kinetics of O2 displacement by CO

✍ Scribed by Lorenzo Cordone; Antonio Cupane

• Publisher: Wiley (John Wiley & Sons)
• Year: 1981
• Tongue: English
• Weight: 311 KB
• Volume: 20
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1981.360201008

✦ Synopsis

Abstract

We studied the kinetics of replacement of O~2~ by CO in hemoglobin in the presence and absence of organic cosolvents (methanol, ethanol, iso‐propanol, n‐propanol, formamide, acetamide, N‐methyl‐formamide) and at 10 and 25°C. Quantitative analysis of the results indicates that these cosolvents do not affect the intrinsic binding constants of ligands to the heme when hemoglobin is in the R conformation. The present results confirm the previously reported suggestion that the effects of the above cosolvents on the oxygen affinity of hemoglobin are related to effects on the T ⇄ R conformational equilibrium.