๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Effect of sodium butyrate on actin distribution in rat 3Y1 fibroblasts in monolayer culture

โœ Scribed by Koji Yamada; Masafumi Sasaki; Genki Kimura

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
898 KB
Volume
125
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

โœฆ Synopsis

We studied the effect of sodium butyrate, a potent GI/G2-arresting agent, on actin distribution in rat 3Y1 fibroblasts in monolayer culture by fluorescence microscopy of cells stained with 7-nitrobenz-2-oxa-I, 3-diazole phallacidine (NBD-Ph). When randomly proliferating cells were arrested mainly in GI phase with butyrate, a reversible overaccumulation of cellular net protein occurred. In the GI-arrested cells, actin markedly accumulated at the margin of cells, and a network structure of actin stress fibers appeared. When density-arrested cells were replated sparsely and rearrested in t h e GI, early s, and G2 phases with butyrate or hydroxyurea, the actin network was observed extensively in the cells arrested in t h e GI and G2 phases with butyrate. These results agree with our previous results indicating the existence of some physiological similarity between cells in the GI and G2 phases and suggest that actin distribution somehow depends on the phases of the cell cycle. The actin profiles observed by t h e NBD-Ph staining were confirmed by transmission electronmicroscopy (TEM) of negatively stained whole cells. TEM further revealed that electron-dense amorphous materials were present at crossing points in the network but rarely present on interconnecting microfilament bundles.

๐Ÿ“œ SIMILAR VOLUMES

Formation of proliferative tetraploid ce
Formation of proliferative tetraploid cells after treatment of diploid cells with sodium butyrate in rat 3Y1 fibroblasts
โœ Koji Yamada; Genki Kimura ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 493 KB

When randomly proliferating rat 3Y1 fibroblasts were treated with sodium butyrate, more than 90% of their cells were arrested reversibly with a 2C DNA content at least 12 h before the GI/S boundary. When cells synchronized in the early S phase were treated with butyrate, approximately 70% of all cel

Effects of serum deprivation on the init
Effects of serum deprivation on the initiation of DNA synthesis in the second generation in rat 3Y1 cells
โœ Atusyuki Okuda; Genki Kimura ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 418 KB

## Abstract Rat 3Y1 cells arrested at early S by hydroxyurea traversed the remainder of S and G2 and completed mitosis after removal of the drug, irrespective of the absence of serum from the culture medium. When cells were deprived of serum for a period between early S and mitosis after removal of

Influence of the deprivation of a single
Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents
โœ Hiroaki Tanaka; Hirokazu Zaitsu; Kazukiyo Onodera; Genki Kimura ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 908 KB

We compared proliferation and survival of various syngeneic transformed cell lines under conditions of depletion of 15 amino acids in Dulbecco-Eagle's medium. We used a normal fibroblast line 3Y1 and 22 transformed sublines of 3Y1 which had been induced by one of seven transforming agents-simian vir