Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents
✍ Scribed by Hiroaki Tanaka; Hirokazu Zaitsu; Kazukiyo Onodera; Genki Kimura
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 908 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
We compared proliferation and survival of various syngeneic transformed cell lines under conditions of depletion of 15 amino acids in Dulbecco-Eagle's medium. We used a normal fibroblast line 3Y1 and 22 transformed sublines of 3Y1 which had been induced by one of seven transforming agents-simian virus 40, mouse polyomavirus, adenovirus type 12, EIA gene of adenovirus type 12, cDNA of Harvey murine sarcoma virus, Rous sarcoma virus, or Nmethyl-N'-nitro-N-nitrosoguanidine. Unlike other untransformed cells examined (mouse BALBIc-3T3 line, mouse NIH-3T3 line, and primary Fischer rat embryo fibroblasts), 3Y1 ceased to proliferate and accumulated in a viable state with a GI-phase DNA content under 14 singular deprivations of amino acid. None of the transformed 3Y1 lines completely arrested in the GI phase of t h e cell cycle and each showed different levels of survival, depending on each transforming agent. As for transformed 3Y1 cells induced by a given virus or a given transforming gene, any one of the three sublines shared the same trend with respect to proliferation and survival. Transformed derivatives induced by N-methyl-N'-nitro-N-nitrosoguanidine showed almost the same trend in proliferation, but the patterns of survival were not uniform. Our observations suggest that the u n i q u e responses of 3Y1 to amino acid depletion are differently modified by different transforming agents.