Shear-induced degradation of plasmid DNA
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C. S. Lengsfeld; T. J. Anchordoquy
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Article
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2002
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John Wiley and Sons
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English
โ 119 KB
The majority of gene therapy clinical trials use plasmid DNA that is susceptible to shear-induced degradation. Many processing steps in the extraction, purification, and preparation of plasmid-based therapeutics can impart significant shear stress that can fracture the phosphodiester backbone of pol