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Effect of serum proteins and osteoblasts on the surface transformation of a calcium phosphate coating: A physicochemical and ultrastructural study

✍ Scribed by Radin, S. ;Ducheyne, P. ;Berthold, P. ;Decker, S.

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 914 KB
Volume: 39
Category: Article
ISSN: 0021-9304
DOI: 10.1002/(sici)1097-4636(199802)39:2<234::aid-jbm10>3.0.co;2-d

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✦ Synopsis

Changes occurring at the surface of a calcium phosphate coating when in contact with osteoblasts versus those in acellular solutions were analyzed. The coating studied is one with a well-documented extensive effect on shortterm bone growth stimulation. Precipitates associated with original crystals and organized in a weblike structure were observed after a 3-week culture with osteoblasts. The precipitates were identified as carbonated hydroxyapatite (c-HA). In contrast, no significant surface changes were detected after immersion in an acellular serum-containing solution. However, in an acellular serum-free solution simulating the ionic composition of plasma, precipitates, identified as c-HA, were abundantly formed. Dissolution of the original coating preceded precipitation. The data support the hypothesis that dissolution of synthetic calcium phosphate ceramics is an initial step in their transformation to a biologically equivalent apatite, and suggest that both solution-mediated (dissolution-precipitation) and cellmediated mechanisms are involved in the surface transformation.

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