Effect of Secondary Structure on the Activity of Enzymes Suspended in Organic Solvents
β Scribed by Aichun Dong; Jeffrey D Meyer; Brent S Kendrick; Mark C Manning; John F Carpenter
- Book ID
- 115566298
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 180 KB
- Volume
- 334
- Category
- Article
- ISSN
- 0003-9861
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π SIMILAR VOLUMES
We studied a model transesterification reaction catalyzed by subtilisin Carlsberg suspended in toluene, n-hexane, diisopropyl ether, and mixtures of these solvents. To account for solvent effects due to differences in water partitioning between the enzyme and the bulk solvents, we measured water sor
A simple methodology has been successfully employed to explain the solvent dependence of the substrate specificity of enzymes in organic media. This methodology, which does not require the knowledge of the enzyme structure and is thus applicable to lyophilized and other noncrystalline enzyme prepara