Simultaneous two-photon excited Β―uorescence (TPF) and second-harmonic generation (SHG) imaging is demonstrated using a single femtosecond laser and a scanning microscope. This composite nonlinear microscopic technique was applied to imaging DNA and chromosomes, and it was shown that the two differen
Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy
β Scribed by Tang, Shuo; Krasieva, Tatiana B.; Chen, Zhongping; Tempea, Gabriel; Tromberg, Bruce J.
- Book ID
- 120639961
- Publisher
- The International Society for Optical Engineering
- Year
- 2006
- Tongue
- English
- Weight
- 198 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1083-3668
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