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Effect of prolonged exposure to organic solvents on the active site environment of subtilisin Carlsberg

✍ Scribed by Vibha Bansal; Yamixa Delgado; Ezio Fasoli; Amaris Ferrer; Kai Griebenow; Francesco Secundo; Gabriel L. Barletta

Book ID
113804633
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
548 KB
Volume
64
Category
Article
ISSN
1381-1177

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πŸ“œ SIMILAR VOLUMES

Effect of crown ethers on structure, sta
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## Abstract Colyophilization or codrying of subtilisin Carlsberg with the crown ethers 18‐crown‐6, 15‐crown‐5, and 12‐crown‐4 substantially improved enzyme activity in THF, acetonitrile, and 1,4‐dioxane in the transesterification reactions of __N__‐acetyl‐L‐phenylalanine ethylester and 1‐propanol a

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We studied a model transesterification reaction catalyzed by subtilisin Carlsberg suspended in toluene, n-hexane, diisopropyl ether, and mixtures of these solvents. To account for solvent effects due to differences in water partitioning between the enzyme and the bulk solvents, we measured water sor

Can conformational changes be responsibl
Can conformational changes be responsible for solvent and excipient effects on the catalytic behavior of subtilisin Carlsberg in organic solvents?
✍ Kai Griebenow; Alexander M. Klibanov πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 117 KB πŸ‘ 3 views

We developed an FTIR (Fourier transform infrared) methodology for quantitatively assessing the secondary structure of proteins suspended in nonaqueous media. This methodology was used to measure the percentages of ␣-helices and ␀-sheets of subtilisin Carlsberg, prepared under different conditions, p