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Effect of Production Method and Gene Amplification on the Glycosylation Pattern of a Secreted Reporter Protein in CHO Cells

✍ Scribed by Matthew L. Lipscomb; Laura A. Palomares; Vanessa Hernández; Octavio T. Ramírez; Dhinakar S. Kompala


Book ID
109387102
Publisher
American Institute of Chemical Engineers
Year
2008
Tongue
English
Weight
215 KB
Volume
21
Category
Article
ISSN
8756-7938

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## Abstract To investigate the effect of culture temperature on erythropoietin (EPO) production and glycosylation in recombinant Chinese hamster ovary (CHO) cells, we cultivated CHO cells using a perfusion bioreactor. Cells were cultivated at 37°C until viable cell concentration reached 1 × 10^7^ c