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Effect of pre-treatment of sperm with progesterone and cholesterol on in-vitro embryo production in cattle

✍ Scribed by G Giritharan; M.K Afsaneh; M Mohan; G Lee; R Rajamahendran

Book ID
117271978
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
61 KB
Volume
51
Category
Article
ISSN
0093-691X

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## Abstract Mouse embryos at day 9 of development were cultured for 24 to 42 h in 50% fetal calf serum and 50% Waymouth's medium containing 0.5 μg/ml insulin supplemented with various amounts of progesterone and estradiol‐17‐β. Unmodified medium contained approximately 0.2% of the normal maternal b