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Effect of polyamines on the uptake of poly (2′-fluoro-2′-deoxyuridylic acid) by a mammalian cell line

✍ Scribed by Janik, Borek ;Sommer, Ronald G.

Publisher
Wiley (John Wiley & Sons)
Year
1976
Tongue
English
Weight
322 KB
Volume
4
Category
Article
ISSN
0091-7419

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✦ Synopsis

Abstract

VERO cells can take up poly(dUfl)^1^ from the medium. The uptake involves surface adsorption and, most probably, intracellular penetration. Part of the poly(dUfl) is hydrolyzed during incubation with the cells but the hydrolysis products are not incorporated into de novo synthesized nucleic acids. The uptake is reduced by serum and stimulated by polycationic ionenes. The magnitude of stimulation depends on the structure of the ionene and the treatment regimen.

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